Aptagen

SS24-PJ-35 Aptamer for Isocarbophos profenofos Aptamer Details

ID# 8387
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Structure
SS24-PJ-35 Aptamer for Isocarbophos profenofos structure
Chemistry
DNA
Target
Isocarbophos
Category
Other
Affinity (Kd)
11.4 µM
Length
35
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dApdGpdCpdTpdTpdGpdCpdTpdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdGpdApdTpdCpdGpdCpdCpdApdCpdApdGpdApdGpdCpdTp-3'
Molecular Weight: 11550
Extinction Coefficient:

Binding Conditions / Buffer

1x Buffer (300 mM NaCl, 50mM KCl, 10 mM MgCl2, and 50 mM Tris)
Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Li WANG, Hua YE, Hong-Qing SANG, Dan-Dan WANG, Aptamer-Based Fluorescence Assay for Detection of Isocarbophos and Profenofos, Chinese Journal of Analytical Chemistry, Volume 44, Issue 5, 2016, Pages 799-803, ISSN 1872-2040, https://doi.org/10.1016/S1872-2040(16)60933-7.
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