Spinach Aptamer Details

ID# 8022

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Structure

Chemistry

RNA

Target

3,5-dimethoxy-4-hydroxybenzylidene imidazolinone (DHMBI)

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprCprUprAprUprUprGprCprUprGprGprAprGprGprGprGprCprGprCprCprAprCprAprUprGprAprAprAprGprUprGprGprUprGprGprUprUprGprGprGprUprGprCprGprGprUprCprGprGprCprGprAprUprAprGprCprUprCp-3'

Molecular Weight:

Extinction Coefficient:

Binding Conditions / Buffer

40 mM HEPES pH 7.4 (or 40 mM Bicine pH 8.0), 125 mM KCl, 5 mM MgCl2, and 5% DMSO

Binding Temp: 23°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA Mimics of Green Fluorescent Protein. Science, 333(6042), 642–646. doi:10.1126/science.1207339

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