SP6 RNA polymerase (S05) Aptamer Details
ID# 7673
Structure
Chemistry
RNA
Target
SP6 RNA Polymerase
Category
Protein
Affinity (Kd)
9.5 nM
Length
35
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rUprUprGprCprUprUprGprGprAprAprUprGprCprGprUprUprAprUprAprGprUprCprUprCprUprUprAprGprGprUprGprUprGprUprAp
Molecular Weight: 11178.67
Extinction Coefficient: 352800
Binding Conditions / Buffer
Binding Buffer (50mM Tris-HCl, pH 7.6, 1 M NaCl, 10mM 2-mercaptoethanol, 10% glycerol) at 4°C overnight at -20°C until use
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mori, Y. (2012). Inhibitory RNA aptamer against sp6 RNA polymerase. Biochemical and Biophysical Resarch Communications, 420 (2012), 440-443.
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