Sp1 Aptamer Details

ID# 8168

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Structure

Chemistry

DNA

Target

Shigella sonnei

Sequence (3-char table code)

G A C U/T

5'-dTpdTpdCpdTpdTpdCpdCpdCpdTpdTpdTpdTpdApdTpdTpdApdGpdTpdCpdCpdTpdGpdTpdApdTpdTpdCpdCpdTpdCpdTpdApdCpdTpdGpdTpdTpdGpdCpdCp-3'

Molecular Weight: 12061.73

Extinction Coefficient: 345300

Binding Conditions / Buffer

50 mM Tris–HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, pH 7.4

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Gong, Wenhui, et al. “Selection, Identification, and Application of Dual DNA Aptamers against Shigella Sonnei.” Analytical Methods, vol. 7, no. 8, 2015, pp. 3625–3631., https://doi.org/10.1039/c5ay00214a.

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