SNAP1 Aptamer SARS-CoV-2 S Protein Aptamer Details

ID# 8565

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Structure

Chemistry

DNA

Target

SARS-CoV-2 Spike Protein

Sequence (3-char table code)

G A C U/T

5'-dTpdCpdGpdCpdTpdCpdTpdTpdTpdCpdCpdGpdCpdTpdTpdCpdTpdTpdCpdGpdCpdGpdGpdTpdCpdApdTpdTpdGpdTpdGpdCpdApdTpdCpdCpdTpdGpdApdCpdTpdGpdApdCpdCpdCpdTpdApdApdGpdGpdTpdGpdCpdGpdApdApdCpdApdTpdCpdGpdCpdCpdCpdGpdCpdGpdTpdApdApdGpdTpdCpdCpdGpdTpdGpdTpdGpdTpdGpdCpdGpdApdAp-3'

Molecular Weight: 26361 g/mole

Extinction Coefficient: 779500

Binding Conditions / Buffer

4.5 g/L glucose, 0.1 g/L CaCl2, 0.2 g/L KCl, 0.2 g/L KH2PO4, 0.1 g/L MgCl2·6H2O, 8 g/L NaCl, 2.1716 g/L Na2HPO4, and 5 mM MgCl2, 1% BSA, 0.1 mg/mL tRNA, 0.1 mg/mL SS DNA, 0.01% Tween-20 @ 25 °C

Binding Temp: 74.4 ºC°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Lucy F. Yang, Nataly Kacherovsky, Nuttada Panpradist, Ruixuan Wan, Joey Liang, Bo Zhang, Stephen J. Salipante, Barry R. Lutz, and Suzie H. Pun. Aptamer sandwich lateral flow assay (AptaFlow) for antibody-free SARS-CoV-2 detection. Analytical Chemistry 2022 94 (20), 7278-7285. DOI: 10.1021/acs.analchem.2c00554

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