SMZ1 Aptamer Details
ID# 8090
Structure
Chemistry
DNA
Target
Sulfamethazine (SMZ)
Category
Small Organic
Affinity (Kd)
167.8 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’- dGpdApdCpdApdGpdGpdCpdApdGpdGpdApdCpdApdCpdCpdGpdTpdApdApdCpdGpdTpdTpdApdGpdApdCpdGpdCpdTpdGpdGpdApdCpdCpdGpdCpdGpdTpdGpdTpdGpdApdTpdCpdGpdGpdGpdTpdCpdApdGpdCpdTpdGpdCpdApdApdTpdTpdCpdTpdGpdCpdTpdApdCpdCpdTpdCpdCpdCpdTpdCpdCpdTpdCpdTpdTpdCp -3'
Molecular Weight: 24543.9
Extinction Coefficient: 738000
Binding Conditions / Buffer
20 mM Tris-HCl, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM
CaCl2, pH 7.4
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kou, Q., Wu, P., Sun, Q., Li, C., Zhang, L., Shi, H., … Le, T. (2020). Selection and truncation of aptamers for ultrasensitive detection of sulfamethazine using a fluorescent biosensor based on graphene oxide. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-020-03044-2
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