Small-Cell Lung Cancer (HCH07) Aptamer Details
ID# 7484
Structure
Chemistry
DNA
Target
Small-cell lung cancer (SCLC) cell-surface molecular markers
Category
Cells
Affinity (Kd)
38 nM
Length
71
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdApdCpdCpdApdGpdTpdGpdCpdGpdApdTpdGpdCpdTpdCpdApdGpdGpdCpdCpdGpdApdTpdGpdTpdCpdApdApdCpdTpdTpdTpdTpdTpdCpdTpdApdApdCpdTpdCpdApdCpdTpdGpdGpdTpdTpdTpdTpdGpdCpdCpdTpdGpdApdCpdGpdCpdApdTpdTpdCpdGpdGpdTpdTpdGpdApdCp-3'
Molecular Weight: 21768.15
Extinction Coefficient: 650200
Binding Conditions / Buffer
Washing Buffer (4.5 g/L Glucose, 5mM MgCl2, and 1 mg/mL BSA in Dulbecco's PBS (pH 7.3)) Binding Buffer (wash buffer and 0.1 mg/mL yeast tRNA)
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chen, Hui, et al. "Molecular Recognition of Small-Cell Lung Cancer Cells Using Aptamers." CHEMMEDCHEM, 3 (2008): 991-1001.
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