SL1025 Aptamer Details
ID# 7973
Structure
Chemistry
Chimeric
Target
Interleukin 6 (IL-6), cytokine
Category
Protein
Affinity (Kd)
0.2 nM
Length
22
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdGpdCpdApdGpdGpdGpdGpdApdApdApdCpdApdCpdGpdApdApdGpdCpdGpdGpdGp
Molecular Weight: 6908.54
Extinction Coefficient: 227300
Binding Conditions / Buffer
PBS with 1%BSA, 0.05% Tween 20, 5 mM MgCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Gupta, Shashi, et al. "Chemically modified DNA aptamers bind interleukin-6 with high affinity and inhibit signaling by blocking its interaction with interleukin-6 receptor." Journal of Biological Chemistry 289.12 (2014): 8706-8719.
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