sk6Ea Aptamer Details
ID# 7998
Structure
Chemistry
DNA
Target
SK-BR-3 cells (HER2 positive)
Category
Cells
Affinity (Kd)
34.79-63.85 nM
Length
53
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdCpdAp dCpdGpdCp dCpdCpdGp dApdTpdAp dApdGpdGp dGpdCpdGp dApdTpdGp dCpdCpdGp dApdTpdCp dCpdCpdTp dGpdTpdGp dGpdCpdCp dGpdTpdAp dGpdGpdGp dCpdApdGp dTpdCpdCp dCpdCpdTp dApdGp-3’
Molecular Weight: 16296.5
Extinction Coefficient: 493200
Binding Conditions / Buffer
PBS (pH 7.4), 5 mM MgCl2, 4.5 g/L glucose, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Liu, M.; Wang, Z.; Tan, T.; Chen, Z.; Mou, X.; Yu, X.; Deng, Y.; Lu, G.; He, N. An Aptamer-Based Probe for Molecular Subtyping of Breast Cancer. Theranostics. 2018, 8, 5772-5783.
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