Aptagen

SE43 Aptamer Details

ID# 8191
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Structure
SE43 structure
Chemistry
DNA
Target
Staphylococcus epidermidis
Category
Cells
Affinity (Kd)
PBS for selection; PBS containing 1% BSA and 0.05% Tween20 for binding assay
Length
76
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdGpdApdTpdCpdCpdGpdTpdGpdApdCpdTpdGpdTpdApdCpdGpdGpdGpdCpdTpdCpdApdGpdTpdCpdGpdTpdTpdApdCpdTpdTpdGpdApdGpdApdGpdTpdTpdGpdApdApdTpdTpdCpdCpdCpdTpdTpdTpdApdGpdTpdGpdApdGpdGpdGpdTpdTp-3'
Molecular Weight: 23501.2
Extinction Coefficient: 729700

Binding Conditions / Buffer

23°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Kaur SJ, Gilman V, Duong M, Asher DM, Gregori L. Rapid selection of single-stranded DNA aptamers binding Staphylococcus epidermidis in platelet concentrates. Biotechniques. 2018 Dec;65(6):331-338. doi: 10.2144/btn-2018-0081. PMID: 30477331.
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