SARS-CoV-2 Variants’ Spike Protein DNA Aptamer K1 Aptamer Details

ID# 8473

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Structure

Chemistry

DNA

Target

Spike Protein of Wild Type SARS-CoV-2 Virus

Sequence (3-char table code)

G A C U/T

5'-dApdApdTpdGpdGpdApdTpdCpdCpdApdCpdApdTpdCpdTpdApdCpdGpdApdGpdCpdTpdCpdGpdApdApdCpdGpdCpdCpdCpdGpdGpdApdGpdGpdApdGpdApdCpdGpdGpdGpdGpdGpdCpdApdGpdGpdGpdCpdGpdTpdGpdTpdTpdCpdApdCpdTpdGpdCpdApdGpdApdCpdTpdTpdGpdApdCpdGpdApdApdGpdCpdTp-3'

Molecular Weight: 25.41 kDa

Extinction Coefficient:

Binding Conditions / Buffer

Binding Buffer containing 20 mM Hepes, 150 mM NaCl, 5 mM KCl, 2 mM MgCl2, 0.5 mM CaCl2, pH 7.4.

Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Le TT, Benton DJ, Wrobel AG, Gamblin SJ. Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants. RSC Adv. 2023 May 19;13(22):15322-15326. doi: 10.1039/d3ra01382k. PMID: 37213341; PMCID: PMC10197177.

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