SARS Coronavirus NTPase/Helicase (ES15-1) Aptamer Details
ID# 7531
Structure
Chemistry
RNA
Target
SARS Coronavirus NTPase/Helicase
Category
Protein
Affinity (Kd)
1.2 nM
Length
107
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprAprUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUprGprCprAprGprAprAprAprAprGprGprGprGprGprAprAprGprAprAprGprAprGprGprGprUprGprAprUprUprCprAprGprGprCprGprAprGprAprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCp-3'
Molecular Weight: 34719.1
Extinction Coefficient: 1104500
Binding Conditions / Buffer
Binding Buffer (30 mM Trisƒ??HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2 , 2 mM dithiothreitol (DTT) and 1% (w/v) BSA) Termination Buffer (0.2 M EDTA, 0.2 M NaCl, and 20 mM HEPES (pH 7.4)) Buffer A (25 mM Trisƒ??HCl (pH 7.5), and 0.3 mM NaCl)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Jang, K., et al. "Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase." Biochemical and Biophysical Research Communications, 366(2008): 738-744.
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