Aptagen

Salmonella Typhimurium OmpC Protein (T-2) Aptamer Details

ID# 7876
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Structure
Salmonella Typhimurium OmpC Protein (T-2) structure
Chemistry
RNA
Target
S. Typhimurium OmpC Protein
Category
Protein
Affinity (Kd)
20 nM
Length
42
Aptazyme
No

Sequence (3-char table code)

G A C U/T
fUpdApdGpfUpdGpfUpdGpdApdGpdApdGpfCpfCpdGpfUpdGpdApdGpfUpdGpdApdApdApdGpdGpfCpfCpdGpfCpdGpdApfCpdApdApdApdGpdApfUpfCpdGpdGpdAp
Molecular Weight: 13276.35
Extinction Coefficient: 429700

Binding Conditions / Buffer

SELEX binding buffer (30 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA)
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Han, S. R. and Lee, S. "In Vitro Selection of RNA Aptamer Specific to Salmonella Typhimurium." Journal of Microbiology and Biotechnology, 23.6(2013): 878-84.
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