Aptagen

Salmonella typhimurium (AptHis) Aptamer Details

ID# 10013
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Structure
Salmonella typhimurium (AptHis) structure
Chemistry
DNA
Target
Salmonella typhimurium
Category
Cells
Affinity (Kd)
1.52 µM
Length
40
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdCpdTpdApdTpdGpdGpdGpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdApdTpdTpdGpdGpdCpdCpdCpdCpdGpdGpdGpdApdGpdCpdTpdGpdGpdCp-3'
Molecular Weight: 12488 g/mole
Extinction Coefficient: 370000

Binding Conditions / Buffer

1x AMP binding buffer (200 mM Tris-Cl, pH 8.8, 200 mM NaOH, and 1 mM MgCl2) equilibrated in 1 × phosphate buffered saline (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4)
Binding Temp: Room temperature°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Ji-Hyun Yeom, Boeun Lee, Daeyoung Kim, Jong-kook Lee, Suk Kim, Jeehyeon Bae, Yoonkyung Park, Kangseok Lee, Gold nanoparticle-DNA aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular Salmonella enterica serovar Typhimurium, Biomaterials, Volume 104, 2016, Pages 43-51, ISSN 0142-9612, https://doi.org/10.1016/j.biomaterials.2016.07.009.
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