Salmonella Enteritidis (S25) Aptamer Details
ID# 7674
Structure
Chemistry
RNA
Target
Salmonella Enteritidis
Category
Cells
Affinity (Kd)
N/A nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGp rUprUprCp rAprCprUp rGprCprAp rGprAprCp rUprUprGp rAprCprGp rAprAprGp rCprUprUp rGprAprGp rAprGprAp rUprGprCp rCprCprCp rCprUprGp rAprUprGp rUprGprCp rAprUprUp rCprUprUp rGprUprUp rGprUprGp rUprUprGp rCprGprGp rCprAprAp rUprGprGp rAprUprCp rCprAprCp rAprUprCp rUprAprCp rGprAprAp rUprUprCp-3'
Molecular Weight: 28847.27
Extinction Coefficient: 875300
Binding Conditions / Buffer
Binding Buffer (0.5 M NaCl, 10 mM Tris-HCl, pH 7.5-7.6, and 1 mM MgCl2 with 0.1% Triton X 100)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hyeon, J. et al. Development of RNA Aptamers for Detection of Salmonellas Enteritidis. Journal of Microbiological Methods, 89(2012), 79-82.
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