S6-1b Aptamer Details
ID# 8136
Structure
Chemistry
DNA
Target
Membrane protein of glioma cell line SHG44
Category
Protein
Affinity (Kd)
3.1-44 nM
Length
53
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dCpdApdCpdApdGpdGpdTpdTpdCpdCpdApdGpdGpdTpdApdApdTpdApdCpdCpdTpdApdApdGpdGpdGpdTpdApdTpdGpdCpdTpdCpdTpdCpdGpdCpdCpdTpdApdTpdTpdApdTpdApdTpdGpdGpdApdGpdCpdApdCp-3’
Molecular Weight: 16291.6
Extinction Coefficient: 513000
Binding Conditions / Buffer
4.5 g/L glucose, 0.01 M PBS, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL BSA.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lin, N.; Wu, L.; Xu, X.; Wu, Q.; Wang, Y.; Shen, H.; Song, Y.; Wang, H., Zhu, Z.; Kang, D.; Yang, C. Aptamer Generated by Cell-SELEX for Specific Targeting of Human Glioma Cells. ACS Appl. Mater. Interfaces. 2021 Mar 3; 13(8): 9306-9315. DOI: 10.1021/acsami.0c11878.
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