S6-1 Aptamer Details
ID# 8061
Structure
Chemistry
DNA
Target
human malignant glioma cell line SHG44
Category
Cells
Affinity (Kd)
44 ± 15 nM
Length
60
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdCpdApdApdGpdTpdCpdApdCpdApdGpdGpdTpdTpdCpdCpdApdGpdGpdTpdApdApdTpdApdCpdCpdTpdApdApdGpdGpdGpdTpdApdTpdGpdCpdTpdCpdTpdCpdGpdCpdCpdTpdApdTpdTpdApdTpdApdTpdGpdGpdApdGpdCpdApdCpdGp-3’
Molecular Weight: 18474
Extinction Coefficient: 583800
Binding Conditions / Buffer
4.5 g/L of glucose, 0.01 M phosphate buffered saline, and 5 mM of MgCl2
Binding Temp: 37 °C°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.
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