S3 Aptamer Details
ID# 8057
Structure
Chemistry
DNA
Target
CD109
Category
Protein
Affinity (Kd)
10.5332 - 13.3326 nM
Length
44
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdCpdTpdGpdApdGpdApdApdTpdApdGpdTpdGpdGpdTpdTpdTpdGpdCpdTpdGpdTpdApdTpdGpdGpdTpdGpdGpdGpdCpdGpdTpdTpdGpdApdApdApdGpdApdGpdGpdGpdGp-3’
Molecular Weight: 13834
Extinction Coefficient: 437600
Binding Conditions / Buffer
Dulbecco’s phosphate buffered saline (D-PBS, Ca2+/Mg2+-free) supplemented with 4.5 g/L of glucose and 5 mM of MgCl2
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Xiong, H., Yan, J., Cai, S., He, Q., Peng, D., Liu, Z., & Liu, Y. (2019). Cancer protein biomarker discovery based on nucleic acid aptamers. International Journal of Biological Macromolecules. doi:10.1016/j.ijbiomac.2019.03.165
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