S22 Aptamer Details
ID# 8157
Structure
Chemistry
DNA
Target
MUC-1
Category
Protein
Affinity (Kd)
0.010 - 1000 nM
Length
25
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdApdGpdTpdTpdGpdApdTpdCpdCpdTpdTpdTpdGpdGpdApdTpdApdCpdCpdCpdTpdGpdGp-3'
Molecular Weight: 7743
Extinction Coefficient:
Binding Conditions / Buffer
Bound to a gold electrode in order to transmit aptasensor data.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wen W., et al. "An insertion approach electrochemical aptasensor for mucin 1 detection based on exonuclease-assisted target recycling." Biosensors and Bioelectronics, 71 (2015): 13–17. doi: https://doi.org/10.1016/j.bios.2015.04.001
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