S21 Aptamer Details
ID# 8065
Structure
Chemistry
DNA
Target
Cas9
Category
Protein
Affinity (Kd)
0.63 nM
Length
47
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdGpdGpdCpdTpdCpdTpdCpdApdCpdApdTpdCpdGpdCpdGpdCpdCpdTpdTpdTpdCpdCpdCpdCpdApdGpdCpdTpdTpdTpdCpdGpdGpdTpdGpdTpdGpdApdGpdApdGpdCpdCpdTpdCp-3'
Molecular Weight: 14334.3
Extinction Coefficient: 418100
Binding Conditions / Buffer
20 mM HEPES, pH 6.5, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
