S14 (SARS-CoV-2 Trimeric S Protein) Aptamer Details

ID# 9673

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Structure

Chemistry

DNA

Target

SARS-CoV-2 Trimeric S Protein

Sequence (3-char table code)

G A C U/T

dTpdGpdGpdGpdApdGpdCpdCpdTpdGpdGpdGpdApdCpdApdTpdApdGpdTpdGpdGpdGpdGpdApdApdApdGpdApdGpdGpdGpdGpdApdApdGpdApdGpdTpdGpdGpdGpdTpdCpdTp

Molecular Weight: 13912 g/mole

Extinction Coefficient: 450500

Binding Conditions / Buffer

1 PBS pH 7.4, 10 mM MgCl2,50 mM KCl, 25 mM NaCl

Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Ankit Gupta, Anjali Anand, Neha Jain, Sandeep Goswami, Anbalagan Anantharaj, Sharanabasava Patil, Rahul Singh, Amit Kumar, Tripti Shrivastava, Shinjini Bhatnagar, Guruprasad R. Medigeshi, Tarun Kumar Sharma, A novel G-quadruplex aptamer-based spike trimeric antigen test for the detection of SARS-CoV-2, Molecular Therapy - Nucleic Acids, Volume 26, 2021, Pages 321-332, ISSN 2162-2531, https://doi.org/10.1016/j.omtn.2021.06.014.

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