S132B-C22 Aptamer Details
ID# 7920
Structure
Chemistry
RNA
Target
Botulinum Neuroxin Type A (BoNT/A)
Category
Protein
Affinity (Kd)
0.8 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rGprAprCprAprGprCprGpUrGprCprCpUrAprGprAprAprGpUrCprCprAprAprGprCpUUrAprAprApUrAprAprCprCprAprCprGprCpUrCprGprAprCprAprAprGprCp
Molecular Weight: 12930.02
Extinction Coefficient: 395100
Binding Conditions / Buffer
PBS, pH 7.4, 300mM NaCl, 3mM MgCl2, 5mM DTT, 1mg/ml BSA, 0.2% Tween, 1mg/ml heparin
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tzuu-Wang Chang, Michael Blank, Pavithra Janardhanan, Bal Ram Singh, Charlene Mello, Michael Blind, Shuowei Cai, "In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin" Biochemical and Biophysical Research Communications 396 (2010) 854ƒ??860.
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