S1 Sars-CoV-2 Apt-126 Aptamer Details

ID# 8446

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Structure

Chemistry

DNA

Target

S1 Protein from SARS-CoV-2

Sequence (3-char table code)

G A C U/T

5'-dGpdCpdCpdApdTpdGpdTpdTpdGpdTpdTpdGpdGpdTpdCpdGpdGpdGpdApdTpdGpdGpdTpdGpdApdApdCpdGpdCpdTpdCpdCpdGpdTpdGpdTpdTpdGpdCpdGp-3'

Molecular Weight: 12422

Extinction Coefficient: 371000

Binding Conditions / Buffer

Aptamer can work in either Buffer 1 or Buffer 2. Buffer 1 contained 25 mM Tris pH 8.0, 150 mM NaCl. Buffer 2 contained 10 mM HEPES pH 7, 150 mM NaCl, 3 mM EDTA, 0.05% Tween 20, 0.5 mg/mL BSA, 0.05 mg/mL salmon sperm DNA.

Binding Temp: 24 °C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Lozoya-Colinas, A., Yu, Y., & Chaput, J. C. (2023b). Functionally enhanced XNA aptamers discovered by parallelized library screening. Journal of the American Chemical Society, 145(47), 25789–25796. https://doi.org/10.1021/jacs.3c09497

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