S. Enterica Serovar Typhi IVP Pili Protein (S-PS8.4) Aptamer Details
ID# 7532
Structure
Chemistry
RNA
Target
S. Enterica Serovar Typhi IVP Pili Protein
Category
Protein
Affinity (Kd)
7.2 nM
Length
71
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprAprCprAprGprUprCprCprGprAprGprCprCprUprCprAprCprUprGprUprUprAprUprCprCprGprAprUprAprGprCprAprGprCprGprCprGprGprGprAprUprGprAprGprGprGprUprCprAprAprUprGprCprGprUprCprAprUprAprGprGprAprUprCprCprCprGprCp-3'
Molecular Weight: 22923.86
Extinction Coefficient: 696500
Binding Conditions / Buffer
Binding Buffer (25 mM Tris-HCl, 50 mM KCl, 200 mM NaCl, 0.2 mM EDTA, 5% [vol/vol] glycerol, 0.5 mM DTT)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Pan, Q., et al. "Aptamers That Preferentially Bind Type IVB Pili and Inhibit Human Monocytic-Cell Invasion by Salmonella enterica Serovar Typhi." Antimicrobial Agents and Chemotherapy, 49(2005): 4052-4060.
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