S-adenosyl homocysteine (CTH-5) Aptamer Details
ID# 7514
Structure
Chemistry
RNA
Target
S-adenosyl homocysteine
Category
Small Organic
Affinity (Kd)
0.1 nM
Length
42
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprCprGprGprAprUprGprAprGprAprCprGprCprUprUprGprGprCprGprUprGprUprGprCprUprGprUprGprGprAprGprAprGprUprCprAprUprCprCprGp-3'
Molecular Weight: 13669.26
Extinction Coefficient: 410600
Binding Conditions / Buffer
RNA Buffer (300 mM NaCl, 25 mM Tris (pH 7.6), 5 mM MgCl2) HBS Buffer (10 mM HEPES (pH 7.4), 150 mM NaCl, 3.4 mM EDTA, and 0.005% P-20) Carbonate Buffer (0.05 M NaHCO3 0.125 M NaCl (pH 8.3))
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Gebhardt, K., et al. "RNA Aptamers to S-Adenosylhomocysteine: Kinetic Properties, Divalent Cation Dependency, and Comparison with Anti-S-adenosylhomocysteine Antibody." Biochemistry, 39 (2000): 7255-7265.
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