S-1 Aptamer Details
ID# 9460
Structure
Chemistry
2'-F-RNA
Target
RNA Helicase Protein DHX9
Category
Protein
Affinity (Kd)
14 µM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
fCprGprGprGpfUpfCprGpfUprApfCprGpfUprAprApfUprGprApfCpfUprAprGpfCprApfCpfCprApfCprAprAprApfCprGprAprApfUpfCprGprGprGpfUp
Molecular Weight: 12240.9 g/mole
Extinction Coefficient: 387700
Binding Conditions / Buffer
radioactive filter-binding assay was performed. Briefly, for aptamer 32P labeling, in vitro transcribed RNA was purified by chromatography, then end-labeled with [γ-32P] ATP using T4 polynucleotide kinase followed by G-50 column purification. Binding assays were performed by incubating 32P-labeled RNA (3 pmol) with the protein at the indicated concentrations in binding buffer.
Binding Temp: 67.5 °C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mi J, Ray P, Liu J, Kuan CT, Xu J, Hsu D, Sullenger BA, White RR, Clary BM. In Vivo Selection Against Human Colorectal Cancer Xenografts Identifies an Aptamer That Targets RNA Helicase Protein DHX9. Mol Ther Nucleic Acids. 2016 Apr 26;5(4):e315. doi: 10.1038/mtna.2016.27. PMID: 27115840; PMCID: PMC5014527.
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