Rous Sarcoma Virus (A) Aptamer Details
ID# 7569
Structure
Chemistry
2'-F-RNA
Target
Rous Sarcoma Virus
Category
Cells
Affinity (Kd)
20 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGpfCpfUpfCprAprGprAprApfUprAprAprApfCprGpfCpfUpfCprAprAprGprGprGpfUprAprGprGprGprApfUpfCprGpfUpfUprApfCpfCpfCpfCprGprApfCprApfUpfUpfUpfUprAprApfUprGprGprGpfCpfCprGprApfUprGpfUpfUpfUpfCprGprApfCprApfUprGprAprGprGpfCpfCpfCprGprGprApfUpfCpfCprGprGpfCp-3'
Molecular Weight: 28169.78
Extinction Coefficient: 861600
Binding Conditions / Buffer
Binding buffer: 2.5 mM MgCl2, 100 mM NaCl, 20 mM Tris-HCl (pH 7.4)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Pan et al. Isolation of virus-neutralizing RNAs from a large pool of random sequences." PNAS, 92(1995): 11509-11513.
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