RNA Tobramycin Molecular Beacon (BA 14-2) Aptamer Details
ID# 7462
Structure
Chemistry
RNA
Target
Tobramycin
Category
Small Organic
Affinity (Kd)
16 µM
Length
104
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprGprGprCprUprUprUprGprAprAprGprGprUprGprAprGprAprCprCprGprUprGprCprAprAprAprUprGprAprGprGprAprUprGprGprUprGprUprGprGprAprUprGprAprUprUprAprGprGprGprUprUprGprUprCprGprGprUprUprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUp-3'
Molecular Weight: 33690.28
Extinction Coefficient: 1052600
Binding Conditions / Buffer
Binding buffer: 50 mM Tris (pH 7.4), 500 mM NaCl
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Morse, Daniel. "Direct selection of RNA beacon aptamers." Biochemical and Biophysical Research Communications, 359 (2007): 94ƒ??101.
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