RNA aptamer-1-based sensitive detection of SARS-CoV nucleocapsid protein Aptamer Details
ID# 8030
Structure
Chemistry
RNA
Target
SARS-CoV Nucleocapsid (N) Protein
Category
Protein
Affinity (Kd)
1.65 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGprCprGprGprAprAprGprCprGprUprGprCprUprGprGprGprCprCprUprGprUprCprGprUprUprCprGprCprUprGprUprGprUprCprUprUprGprCprUprAprCprGprUprUprAprCprGprUprUprAprCprAprCprGprGprUprUprGprGprCprAprUprAprAprCprCprCprAprGprAprGprGprUprCprGprAprUprGprGp-3'
Molecular Weight: 20566.55
Extinction Coefficient: 1021300
Binding Conditions / Buffer
30mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 2mM DTT, 1%BSA
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ahn, Dae-Gyun,et al. "RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein" The Royal Society of Chemistry, (2009):1896-1901.
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