RNA 14-16 Aptamer Details
ID# 9005
Structure
Chemistry
RNA
Target
p68 oncogenic helicase
Category
Protein
Affinity (Kd)
13.8 nM
Length
55
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rGprGprGprAprGprGprAprCprGprAprUprGprCprGprGprCprAprGprUprGprCprCprCprAprAprCprCprGprGprAprAprCprAprAprCprAprAprCprCprAprCprCprGprGprCprGprGprCprUprCprCprUprGprCprUp
Molecular Weight: 16894.9 g/mole
Extinction Coefficient: 514600
Binding Conditions / Buffer
Biotinylated RNA motifs immobilized on streptavidin
magnetic beads were incubated with tumor tissue extracts and
washed. RNA-bound proteins were then eluted in high salt solution and resolved electrophoretically before Coomassie blue staining .
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mi J, Liu Y, Rabbani ZN, Yang Z, Urban JH, Sullenger BA, Clary BM. In vivo selection of tumor-targeting RNA motifs. Nat Chem Biol. 2010 Jan;6(1):22-4. doi: 10.1038/nchembio.277. Epub 2009 Nov 29. PMID: 19946274; PMCID: PMC2795795.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
