Aptagen

Ricin A-chain (RTA) Aptamer (RA80.1.d2 – 31RA) Aptamer Details

ID# 7953
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Structure
Ricin A-chain (RTA) Aptamer (RA80.1.d2 – 31RA) structure
Chemistry
RNA
Target
Ricin A-chain (RTA)
Category
Protein
Affinity (Kd)
7.4 nM
Length
31
Aptazyme
No

Sequence (3-char table code)

G A C U/T
rGprGprCprGprAprAprUprUprCprAprGprGprGprGprAprCprGprUprAprGprCprAprAprUprGprAprCprUprGprCprCp
Molecular Weight: 8089.29
Extinction Coefficient: 261800

Binding Conditions / Buffer

1X PBS, 5 mM MgCl2
Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

(1) Bogomolova, A., Aldissi, M. ƒ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.ƒ? Biosensors and Bioelecgtronics, 66 (2015): 290-296. (2) Hesselberth, J., et al. ƒ??In vitro Selection of RNA Molecules That Inhibit the Activity of Ricin A-chain.ƒ? Journal of Biological Chemistry, 275, 7 (2000): 4937-4942.
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