RhD antigen‑specificity 2 Aptamer Details
ID# 8106
Structure
Chemistry
DNA
Target
RhD+ red blood cells
Category
Cells
Affinity (Kd)
737.7±161.8 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdTpdApdGpdCpdApdGpdCpdCpdCpdCpdGpdCpdGpdGpdApdGpdGpdGpdTpdCpdGpdGpdCpdTpdApdTpdApdApdGpdApdApdCpdCpdApdApdGpdAp-3'
Molecular Weight: 12430.1
Extinction Coefficient: 399400
Binding Conditions / Buffer
10 mM Tris-HCl, 50 mM KCl, pH 9.0), 1.5 mM MgCl2, 400 µM each dNTP, 2.5 U Taq DNA polymerase (Promega Corporation), and 50 pmol each biotinylated primer
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
'
Zhang Y, Xu H, Wang X, Wang L, Liu R, Li L, Zhou H. Single‑strained DNA aptamers mask RhD antigenic epitopes on human RhD+ red blood cells to escape alloanti‑RhD immunological recognition. Mol Med Rep. 2020 Apr;21(4):1841-1848. doi: 10.3892/mmr.2020.10985. Epub 2020 Feb 12. PMID: 32319623; PMCID: PMC7057830.
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