RhD antigen‑specificity 1 Aptamer Details
ID# 8105
Structure
Chemistry
DNA
Target
RhD+ red blood cells
Category
Cells
Affinity (Kd)
580.5±142.0 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdCpdCpdTpdGpdGpdTpdCpdTpdGpdTpdTpdApdGpdCpdCpdGpdGpdGpdTpdApdGpdCpdApdGpdCpdCpdCpdCpdGpdGpdCpdApdCpdCpdTpdApdTpdTp-3'
Molecular Weight: 12280.9
Extinction Coefficient: 363800
Binding Conditions / Buffer
10 mM Tris-HCl, 50 mM KCl, pH 9.0), 1.5 mM MgCl2, 400 µM each
dNTP, 2.5 U Taq DNA polymerase (Promega Corporation), and 50 pmol each biotinylated primer
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Zhang Y, Xu H, Wang X, Wang L, Liu R, Li L, Zhou H. Single‑strained DNA aptamers mask RhD antigenic epitopes on human RhD+ red blood cells to escape alloanti‑RhD immunological recognition. Mol Med Rep. 2020 Apr;21(4):1841-1848. doi: 10.3892/mmr.2020.10985. Epub 2020 Feb 12. PMID: 32319623; PMCID: PMC7057830.
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