Rev Peptide Aptamer Details
ID# 7649
Structure
Chemistry
RNA
Target
Rev Peptide
Category
Peptide
Affinity (Kd)
18 µM
Length
77
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprCprCprUprCprGprGprCprGprAprAprAprGprCprCprGprGprUprAprAprCprGprCprCprAprCprAprAprGprUprCprGprGprAprGprGprGprUprAprAprGprAprUprCprUprGprAprCprAprGprGprAprAprCprUprGprGprGprUprGprAprCprGprGprAprGprGprUprUprAprGprGprUprGprCp-3'
Molecular Weight: 25129.29
Extinction Coefficient: 774600
Binding Conditions / Buffer
Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2), r. t.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hesselberth et al. "Simultaneous detection of diverse analytes with an aptazyme ligase array." Analytical Biochemistry, 312(2003): 106-112.
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