Rd29C33-T7 Aptamer Details
ID# 8070
Structure
Chemistry
DNA
Target
Melamine
Category
Small Organic
Affinity (Kd)
0.92-0.98 nM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dGpdCpdAp dCpdApdCp dCpdGpdAp dTpdGpdGp dCpdGpdGp dTpdCpdCp dTpdGpdTp dTpdTpdAp dGpdGpdGp dGpdGpdTp dGpdTpdGp dCp -3'
Molecular Weight: 10546.8
Extinction Coefficient: 317300
Binding Conditions / Buffer
Cyanuric acid, Ammeline, Ammelide.
Binding Temp: 59°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Gu, Chunmei et al. “Label-free fluorescence detection of melamine with a truncated aptamer.” The Analyst vol. 141,14 (2016): 4511-7. doi:10.1039/c6an00537c
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