RBP4 binding aptamer (RBA) Aptamer Details

ID# 7773

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Structure

Chemistry

DNA

Target

Retinol-binding protein-4 (RBP4)

Sequence (3-char table code)

G A C U/T

5'-dApdTpdApdCpdCpdApdGpdCpdTpdTp dApdTpdTpdCpdApdApdTpdTpdApdCp dApdGpdTpdApdGpdTpdGpdApdGpdGp dGpdGpdTpdCpdCpdGpdTpdCpdGpdTp dGpdGpdGpdGpdTpdApdGpdTpdTpdGp dGpdGpdTpdCpdGpdTpdGpdGpdApdGp dApdTpdApdGpdTpdApdApdGpdTpdGp dCpdApdApdTpdCpdTp-3'

Molecular Weight: 23679.45

Extinction Coefficient: 755800

Binding Conditions / Buffer

Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6.

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.

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