Ras DNA Aptamer Ra1 Aptamer Details

ID# 8405

Back to Search Results

Structure

Chemistry

DNA

Target

Human Ras Protein

Sequence (3-char table code)

G A C U/T

5'-dGpdApdTpdCpdCpdGpdGpdGpdCpdCpdTpdCpdApdTpdGpdTpdCpdGpdApdApdCpdCpdApdCpdGpdCpdApdCpdApdCpdTpdCpdApdCpdTpdApdApdApdCpdCpdGpdCpdGpdTpdCpdGpdApdTpdCpdCpdGpdApdCpdGpdApdTpdTpdGpdApdGpdCpdGpdTpdTpdTpdApdTpdTpdCpdTpdGpdApdGpdCpdTpdCpdCpdCp-3'

Molecular Weight: 51.48 kDa

Extinction Coefficient:

Binding Conditions / Buffer

20 mM HEPES, pH 7.35, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

[1] Ling N, Liu H, Guo J, Liang Z, Zhang Y, Li H, Wu H, Xie T, Yuan Y, Li X, Peng M, Wei X, Liang L, Liu J, Wu W, Ye M. Generation of DNA Aptamers with Functional Activity in Mammalian Cells by Mimicking Retroviruses. Anal Chem. 2023 Jul 4;95(26):9797-9804. doi: 10.1021/acs.analchem.3c00387. Epub 2023 Jun 16. PMID: 37327388. [2] Wang ZW, Wu HB, Mao ZF, Hu XP, Zhang H, Hu ZP, Ren ZL. In vitro selection and identification of ssDNA aptamers recognizing the Ras protein. Mol Med Rep. 2014 Sep;10(3):1481-8. doi: 10.3892/mmr.2014.2337. Epub 2014 Jun 16. PMID: 24938205.

Request a Quote Have your aptamer oligo synthesized by Aptagen.