RA16 Aptamer Details
ID# 8056
Structure
Chemistry
RNA
Target
NCl-H460
Category
Cells
Affinity (Kd)
24.75 ± 2.28 nM
Length
83
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- rGprGprGprAprGprAprGprAprAprCprAprAprUprGprAprCprCprUprGprCprGprGprUprGprCprCprAprAprGprCprCprGprUprCprGprGprGprUprUprAprUprGprUprUprGprAprUprCprUprCprCprUprCprAprAprGprGprAprCprGprAprGprUprGprCprAprUprUprGprCprAprUprCprAprCprGprUprCprAprGprUprAprGp-3'
Molecular Weight: 26830.1
Extinction Coefficient: 826700
Binding Conditions / Buffer
10× transcription buffer (400 mM Tris-Cl, 80 mM MgCl2, and 20 mM spermidine), 10 mM dithiothreitol, 20 U T7 mutant (Y639F) RNA polymerase, 10 mM ATP, 10 mM GTP, 10 mM 2′-F-dCTP/UTP, 2 mM 16-Biotin-UTP, 20 U RiboLock RNase Inhibitor, and 0.05 U inorganic pyrophosphatase. The resulting reaction mixture was treated with 2 μL DNase I at 37 °C for 1 h, followed by phenol-chloroform extraction.
Binding Temp: 37 °C°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wang, Hanlu, et al. “In Vivo SELEX of an Inhibitory NSCLC-Specific RNA Aptamer from PEGylated RNA Library.” Molecular Therapy - Nucleic Acids, Cell Press, 9 Dec. 2017, www.sciencedirect.com/science/article/pii/S2162253117303013.
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