R5A1 Aptamer Details
ID# 9101
Structure
Chemistry
RNA
Target
interleukin-10 receptor
Category
Protein
Affinity (Kd)
12 nM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rCprUprGprUprAprAprUprUprGprGprCprGprUprAprUprGprUprAprUprGprUprAprAprCprCprCprAprGprGprCprAprCprCprAprAprAprCprAprCprCprCprCprUprUprAprUprUprGprUprGprGprCprAprCprCp
Molecular Weight: 10823.6 g/mole
Extinction Coefficient: 329400
Binding Conditions / Buffer
Purified RNA was folded by heating to 82 °C 5′ in selection buffer (150 mmol/l NaCl, 20 mmol/l KCl, 20 mmol/l HEPES) and cooling slowly to room temperature
Binding Temp: 37 °C°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Berezhnoy A, Stewart CA, Mcnamara JO 2nd, Thiel W, Giangrande P, Trinchieri G, Gilboa E. Isolation and optimization of murine IL-10 receptor blocking oligonucleotide aptamers using high-throughput sequencing. Mol Ther. 2012 Jun;20(6):1242-50. doi: 10.1038/mt.2012.18. Epub 2012 Mar 20. PMID: 22434135; PMCID: PMC3369303.
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