Aptagen

R3(2) – truncated Aptamer Details

ID# 8205
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Structure
R3(2) – truncated structure
Chemistry
DNA
Target
immunoglobulin E
Category
Antibody
Affinity (Kd)
22.6 nM
Length
38
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dCpdGpdCpdCpdCpdGpdTpdGpdTpdTpdTpdApdTpdCpdCpdGpdTpdTpdTpdGpdCpdTpdCpdTpdTpdApdGpdTpdGpdGpdCpdApdCpdGpdGpdGpdGpdGp-3'
Molecular Weight: 11778.57
Extinction Coefficient:

Binding Conditions / Buffer

1× PBS containing 2 mM MgCl2 - (PBSM)
Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Bai, cheng, et al." Surface acoustic wave‑assisted microfluidic isolation of aptamers." Microfluidics and Nanofluidics, 26(2002): 1-10
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