R12 Aptamer Details

ID# 8217

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Structure

Chemistry

RNA

Target

Bovine PrP; beta Bovine PrP

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprGprAprGprGprAprGprGprAprGprGprAp-3'

Molecular Weight: 3824.5 g/mole

Extinction Coefficient: 135500

Binding Conditions / Buffer

20 mM Tris-HCl (pH 7.5) and 100 mM NaCl

Binding Temp: Selection at room temperature°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Tsukasa Mashima, Akimasa Matsugami, Fumiko Nishikawa, Satoshi Nishikawa, Masato Katahira, Unique quadruplex structure and interaction of an RNA aptamer against bovine prion protein, Nucleic Acids Research, Volume 37, Issue 18, 1 October 2009, Pages 6249–6258, https://doi.org/10.1093/nar/gkp647 Murakami, K., Nishikawa, F., Noda, K., Yokoyama, T., & Nishikawa, S. (2008). Anti-bovine prion protein RNA aptamer containing tandem GGA repeat interacts both with recombinant bovine prion protein and its β isoform with high affinity. Prion, 2(2), 73–80. https://doi.org/10.4161/pri.2.2.7024

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