R10C5 Aptamer Details
ID# 8114
Structure
Chemistry
DNA
Target
Legionella pneumophila (Lp)
Category
Other
Affinity (Kd)
116 nM
Length
69
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdGpdGpdApdCpdApdGpdTpdGpdCpdTpdGpdApdApdApdApdCpdTpdGpdTpdGpdApdCpdCpdCpdCpdCpdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
Molecular Weight: 21213.8
Extinction Coefficient: 658800
Binding Conditions / Buffer
1X PBS with 0.1 mg/ml salmon sperm DNA, 1% bovine serum albumin, and 0.05% Tween 20
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Saad M, Chinerman D, Tabrizian M, Faucher SP. Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity. Sci Rep. 2020 Jun 4;10(1):9145. doi: 10.1038/s41598-020-65973-3. PMID: 32499557; PMCID: PMC7272621.
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