R10C1 Aptamer Details
ID# 8121
Structure
Chemistry
DNA
Target
Legionella pneumophila (Lp)
Category
Other
Affinity (Kd)
135 nM
Length
60
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdCpdCpdApdCpdCpdCpdCpdApdCpdGpdCpdTpdGpdCpdTpdCpdCpdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
Molecular Weight: 18260.8
Extinction Coefficient: 550100
Binding Conditions / Buffer
1X PBS with 0.1 mg/ml salmon sperm DNA, 1% bovine serum albumin, and 0.05% Tween 20.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Saad, M.; Chinerman, D.; Tabrizian, M.; Faucher, S. Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity. Sci Rep. 2020 Jun 4; 10: 9145. doi: 10.1038/s4598-020-65973-3. PMCID: PMC7272621. PMID: 32499557.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
