PSMA Dimeric Aptamer Aptamer Details
ID# 8027
Structure
Chemistry
DNA
Target
Prostate Specific Membrane Antigen (PSMA)
Category
Protein
Affinity (Kd)
SELEX forward rounds were performed by adding 1 mg of PSMA bound to Dynabeads Talon (Invitrogen, Oslo, Norway) to 700 μl of binding buffer (100 mmol/l NaCl, 20 mmol/l Tris, 2 mmol/l MgCl2, 5 mmol/l KCl, 1 mmol/l CaCl2, 0.2% Tween-20, pH 8). The beads were removed using a Dynal magnet (Invitrogen) and were washed four times with binding buffer. At least 10 μg of ssDNA was annealed by heating to 95 °C followed by gentle cooling and was added to the PSMA matrix followed by vortexing and incubation for 1 hour at 37 °C with mild agitation.
Length
72
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdGpdTpdTpdTpdTpdCpdGpdCpdTpdTpdTpdTpdGpdCpdGpdTpdTpdTpdTpdGpdGpdGpdTpdCpdApdTpdCpdTpdGpdCpdTpdTpdApdCpdGpdApdTpdApdGpdCpdApdApdTpdGpdCpdTpdCpdGpdGpdCpdApdApdApdApdApdApdApdApdApdApdApdApdApdApdApdApdGpdCpdCpdGp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
25°C
Binding Temp: At least 10 μg of ssDNA was annealed by heating to 95 °C followed by gentle cooling and was added to the PSMA matrix followed by vortexing and incubation for 1 hour at 37 °C with mild agitation.
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Boyacioglu, O., Stuart, C. H., Kulik, G., & Gmeiner, W. H. (2013). Dimeric DNA Aptamer Complexes for High-capacity-targeted Drug Delivery Using pH-sensitive Covalent Linkages. Molecular therapy. Nucleic acids, 2(7), e107. https://doi.org/10.1038/mtna.2013.37
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
