PSMA Aptamer (A10-3) Aptamer Details
ID# 7498
Structure
Chemistry
2'-F-RNA
Target
PSMA
Category
Protein
Affinity (Kd)
20.5 nM
Length
56
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGprApfUpfCprAprGpfCpfCprApfUprGpfUpfUpfUprApfCprGpfUpfCprApfCpfUpfCpfCpfUpfUprGpfUpfCprAprApfUpfCpfCpfUpfCprApfUpfCprGprGpfCp-3'
Molecular Weight: 17966.62
Extinction Coefficient: 539200
Binding Conditions / Buffer
HEPES buffered saline (pH 7.4), 1 mM MgCl2, 1 mM CaCl2, 0.01% I-block (Tropix Inc. Bedford, MA), and 0.05% Tween 20. Incubated at 37 °C for 30 minutes. Washed using the above buffer at 37 °C.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lupold, Shawn, et al. "Identification and Characterization of Nuclease-stabilized RNA Molecules That Bind Human Prostate Cancer Cells via the Prostate-specific Membrane Antigen." American Association for Cancer Research Journal 62, (2002): 4029-4033
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