Aptagen

PSA-1 Aptamer Details

ID# 8202
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Structure
PSA-1 structure
Chemistry
DNA
Target
PSA (glycosylated)
Category
Protein
Affinity (Kd)
177 +/-65 nM
Length
40
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdApdCpdGpdGpdTpdTpdGpdCpdGpdCpdTpdApdTpdApdTpdTpdTpdApdApdCpdCpdApdApdApdApdGpdTpdCpdTpdGpdGpdApdTpdTpdApdApdCpdAp-3'
Molecular Weight: 12422.09
Extinction Coefficient:

Binding Conditions / Buffer

PBS
Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Diaz-Fernandez, A, et al. "Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward a more specific detection of prostate cancer." Biosensors and Bioelectronics, 128 (2019): 83-90. doi: https://doi.org/10.1016/j.bios.2018.12.040
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