PrP Fibrils (SAF-93) Aptamer Details

ID# 7487

Back to Search Results

Structure

Chemistry

2'-F-RNA

Target

PrP Fibrils

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprApfCprAprAprGprApfCpfUprAprGprApfCprGpfCpfUpfCprAprApfCpfUprApfCprGprAprApfCpfUpfCprApfUprGprApfCprApfCprAprAprGprGprApfUprGpfCprAprApfUpfCpfUpfCprApfUpfCpfCpfCprGpfCpfCprAprGpfCpfCpfCprApfCpfCprGpfUpfUpfUpfCprGprApfCprApfUprGprAprGprApfCpfUpfCprApfCprAprApfCprAprGpfUpfUpfCpfCpfCpfUpfUpfUprAprGpfUprGprAprGprGprGpfUpfUprAprApfUpfUp-3'

Molecular Weight: 37278.21

Extinction Coefficient: 1146800

Binding Conditions / Buffer

Binding Buffer (20 mM Hepes (pH 7.2), 100 mM NaCl, 50 mM KCl, 10 mM MgCl2)

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Rhie, A., et al. "Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion." Journal of Biological Chemistry, 278 (2003): 39697-705. https://doi.org/10.1074/jbc.M305297200 Mollasalehi N., Francois-Moutal L., Porciani D., Burke D.H., & Khanna M. (2022). Aptamers Targeting Hallmark Proteins of Neurodegeneration. Nucleic Acid Therapeutics, 32(4). https://doi.org/10.1089/nat.2021.0091

Request a Quote Have your aptamer oligo synthesized by Aptagen.