ProApt-15 Aptamer Details

ID# 9308

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Structure

Chemistry

DNA

Target

Procymidone (a dicarboximide fungicide)

Sequence (3-char table code)

G A C U/T

5'-dTpdApdGpdGpdGpdApdApdTpdTpdCpdGpdTpdCpdGpdApdCpdGpdGpdApdTpdCpdCpdApdTpdGpdGpdCpdApdCpdApdTpdGpdGpdApdTpdApdCpdTpdApdGpdCpdGpdApdApdCpdGpdCpdApdTpdGpdGpdGpdCpdGpdGpdCpdCpdGpdCpdGpdGpdCpdGpdCpdApdTpdGpdCpdGpdTpdCpdGpdApdCpdCpdTpdGp-3'

Molecular Weight: ~23.8 kDa

Extinction Coefficient:

Binding Conditions / Buffer

Buffer: 1×BB buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl₂, pH 7.4) Incubation: 1 hr at RT with target; 2 h GO incubation for fluorescence assay Denaturation/Refolding: 95 °C for 10 min → 60 °C for 1 min → 4 °C for 5 min (cooling at 0.1 °C/s)

Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Dong, H., Wang, H., Guo, Z., Huang, J., Zhang, P., Guo, Y., & Sun, X. (2024). Combination of Capture-SELEX and Post-SELEX for procymidone-specific aptamer selection and broad-specificity aptamer discovery, and development of aptamer-based lateral flow assay. Analytica Chimica Acta, 1318, 342922. https://doi.org/10.1016/j.aca.2024.342922

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