Pro-Gastrin Releasing Peptide Aptamer Details
ID# 7836
Structure
Chemistry
DNA
Target
Pro-gastrin releasing peptide (proGRP)
Category
Protein
Affinity (Kd)
1.2 µM
Length
61
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTp dCpdApdApdTpdTpdTpdGpdCpdApdCpdCpdApdCpdTpdTpdApdTpdTpdGpdTpdTpdApdCpdTpdApdApdTpdCpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Molecular Weight: 18685.27
Extinction Coefficient: 593700
Binding Conditions / Buffer
SELEX buffer used in SPR binding assay: 137 mM NaCl, 8.1 mM Na2HPO4, 2.68 mM KCl, 1.47 mM KH2PO4, 0.9 mM CaCl2, 5.3 mM MgCl2, and 4.5 g/L glucose (pH 7.4)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
M Mie et al. (2012) Selection of DNA aptamers with affinity for Pro-gastrin-Releasing Peptide (proGRP), a tumor marker for small cell lung cancer. Appl. Biochem. Biotechnol. Published Online ahead of Print: DOI 10.1007/s12010-012-9956-5
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