Platelet-Derived Growth Factor (PDGF) B-chain (36t) Aptamer Details
ID# 7738
Structure
Chemistry
DNA
Target
Platelet-Derived Growth Factor -BB (PDGF-BB)
Category
Protein
Affinity (Kd)
0.093 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdApdCpdApdGpdGpdCpdTpdApdCpdGpdGpdCpdApdCpdGpdTpdApdGpdApdGpdCpdApdTpdCpdApdCpdCpdApdTpdGpdApdTpdCpdCpdTpdGpdTpdGpdTi-3'
Molecular Weight: 12266
Extinction Coefficient: 382300
Binding Conditions / Buffer
Selection Buffer: PBSM(10.1 mM Na2HPO4, 1.8 mM K2HPO4, 137 mM NaCl and 2.7 mM KCl, 1 mM MgCl2 (pH 7.4))
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Green, L., et al. "Inhibitory DNA Ligands to Platelet-Derived Growth Factor B-Chain." Journal of Biochemistry, 35(1996): 14413-14424.
Stejskalov?, Anna & Oliva, Nuria & J England, Frances & Almquist, Benjamin. (2019). Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces. Advanced Materials. 1806380. 10.1002/adma.201806380.
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